滚球体育

复纳科学仪器(上海)有限公司
技术文章
您现在所在位置:滚球体育 > 技术中心 > 扫描电镜生物样品常用制备方法

扫描电镜生物样品常用制备方法

 发布时间:2019-11-01 点击量:420

SAOMIAODIANJINGZAIGUANCHASHENGWUYANGPINSHI,JUYOUYIXIATEDIAN:DUOJIAODUGUANCHAYANGPINDEBIAOMIANJIEGOU;BUXUYAOJIANGYANGPINQIECHENGBOPIAN;JINGSHENDA、TUXIANGLITIGANQIANG;FANGDABEISHUCONGJISHIBEIDAOJISHIWANBEILIANXUKEDIAO;ZAIGUANCHAXINGMAODETONGSHIKEYIDUIWEIQUDECHENGFENJINXINGDINGLIANGHEDINGXINGFENXI。ERNENGFOUHUODEZHENSHI、QINGXI、LIXIANGDESAOMIAODIANJINGGUANCHAJIEGUO,YANGPINDEZHIBEIGUOCHENGSHIGUANJIAN。

 

SHENGWUYANGPINHANSHUIZHIJIEGUANCHA,HUIDUISAOMIAODIANJINGZAOCHENGYIXIAYINGXIANG:

 

1. 样品蒸发的水蒸气遭遇高能电子束,会被电离而放电,引起束流大幅度波动,使图像模糊,或者根本不能成像;
2. 大多数含水样品在高真空中容易发生形态损伤,表面皱缩、变形;
3. 样品挥发会造成镜头、光阑等的污染;
滚球体育4. 灯丝会被上升水蒸气氧化而变质。

 

SHENGWUYANGPINDEHANSHUILIANGGAO,ERCIDIANZICHANLVDI,DAODIANXINGCHA,DUIRE,DIANZISHUMINGAN,JINYOUSHAOSHUYANGPINRUMAOFA、YACHIYIJIHANSHUILIANGJIDIDEKUNCHONGDENGKEYIZHIJIEPENDUGUANCHA,JUEDADUOSHUDESHENGWUYANGPINJUNYAOQIUJINGGUOGANZAOCHULICAINENGDUJINGUANCHA。

 

GANZAOSHISAOMIAODIANJINGSHENGWUYANGPINZHIBEIZHONGDEGUANJIANHUANJIE,RUGUOCHULIBUHAO,HUIZHIJIEYINGXIANGDAOGUANCHADEQINGXIDUYUZHUNQUEDU。YAOXIANGZHIBEICHUHAODESAOMIAODIANJINGSHENGWUYANGPIN,XUYAOZAIGANZAOGUOCHENGZHONGJINKENENGJIANSHAOYOUYUSHUIFENZHENGFAERYINQIDEYANGPINBIAOMIANXINGMAODEXINGBIAN,QIEBIXUQUEBAOGANZAOCHEDI。DADUOSHUDONGZHIWURONGYIFASHENGMINGXIANDETAXIANHEBIANXING。YINCI,XUYAOZHENDUIBUTONGDESHENGWUYANGPINLAIXUANZEHESHIDEGANZAOFANGFA。

 

扫描电镜生物样品制备常用干燥方法

 

1. 自然干燥法

 

滚球体育ZIRANGANZAOFASHIZHIYANGPINZHONGDESHUIFENZAIDAQIZHONGZIRANZHENGFA,HUOYANGPINJINGTUOSHUICHULIHOUTUOSHUIJIZIRANHUIFAERGANZAODEFANGFA。

 

DUIGANZHONGZI、GUOQIAO、MOUXIEGANHUAFEN、KUNCHONGBIAOBENDENGLAISHUO,ZIRANGANZAOFASHIYIGEJIANYISHIYONGYOUXIAODEFANGFA,SUIRANZAIZIRANGANZAOGUOCHENGZHONG,YANGPINTIJIYOUSUOSHOUSUO,DANQUEBAOLIULEYANGPINDEJIBENXINGTAI。SHIYONGYUWAIBIAOJIANYINGDEYANGPIN,RUWAIBIAOYOUQIAODEKUNCHONG,MUCAIDENG。

 

 滚球体育  

ZIRANFENGGAN——FEINADIANJINGZHIJIEGUANCHAMANCHONG

 

2. 烘干干燥法

 

HONGGANGANZAOFASHIJIANGYAOYANJIUDEYANGPINYONGHONGGANXIANGHONGGAN,YIBANWENDUKONGZHIZAI 80℃ YIXIA,HONGGANCHENGDUYIHANSHUILIANGZAI 5% YIXIAWEIHAO。CIFANGFADEGANZAOSUDUJIAOKUAI,DANSHUIFENZHENGFASHIKENENGZAOCHENGYANGPINBIANXINGHUODUANLIE。SHIYONGYUBUYIBIANXINGQIENAIREDEYANGPIN,BIRUDIANFENLI、BAOZIFENDENG。

 

 滚球体育  

HONGGANDEHUAFENPENJINHOUZHIJIEKEYIYONGERCIDIANZIGUANCHA

 

3. 临界点干燥法

 

滚球体育LINJIEDIANGANZAOFASHILIYONGWUZHIZAILINJIEZHUANGTAIXIA,YETIHEQITIDEMIDUXIANGDENG,QIYEJIEMIANWANQUANXIAOSHI,YETIDEBIAOMIANZHANGLIXISHUWEILING。LINJIEDIANGANZAOFAZHISUOYIYIZHIBEISHIWEIZHIZUOSHENGWUYIXUESAOMIAOYANGPINKEKAODEGANZAOFANGFA,SHIYINWEICIFANENGXIAOCHUYETIBIAOMIANZHANGLIDEZUOYONG,GANZAOCHUDEYANGPINNENGzuiDACHENGDUDIBAOCUNQIZIRANXINGTAI。

 

4. 冷冻干燥法

 

滚球体育LENGDONGGANZAOFASHIJIANGHANSHUIYANGPINFANGRUDIWENHUANJINGZHONGLENGDONG,HUOSHIYONGRONGJIJIANGYANGPINZHONGDESHUIZHUJITIHUANHOULENGDONG,RANHOUCHOUZHENKONGSHENGHUA。TASHILIYONGDIWENHEZHENKONG,SHIYANGPINZHONGDESHUIFENHUORONGJIZHIJIESHENGHUA,YIDADAOGANZAOYANGPINDEMUDE。LENGDONGGANZAOGUOCHENGBUJINGGUOYEXIANGJIEDUAN,YINERBIMIANLEQIXIANGHEYEXIANGZHIJIANBIAOMIANZHANGLIDUIYANGPINDESUNSHANG。

 

5. 真空干燥法

 

ZHENKONGGANZAOFAZHIDESHIJIANGJINGTUOSHUI(DUOYONGTIDUTUOSHUI)DEYANGPINZHIYUZHENKONGRONGQIZHONGJINXINGGANZAODEFANGFA。ZHENKONGGANZAOFAXUANYONGGAORONGDIANDEYOUJIRONGJI(SHUDINGCHUN、YIJING、LIUJIAJIERGUIANWAN、ZHENGDINGCHUNDENG)ZUOSHENGHUAJIEZHI,JIBAOLIULELENGDONGGANZAOFADEYOUDIAN,YOUBUYONGDUIYANGPINJINXINGLENGDONGCHULI,WULENGDONGSUNSHANG,QIECAOZUOJIANDAN。SHIYONGYUSUOYOUSHENGWUYANGPIN,TEBIESHIXIJUN、XIBAODENGWEIXIAOYANGPIN。

 

    

JINGGUOTIDUTUOSHUIZHENKONGGANZAODEGANJUNKEYIQINGSONGHUODEGAOBEITUXIANG

 

小结

 

GANZAOFANGFAZAIBUDUANGAIJIN,DANHAISHIZHINENGBAYOUGANZAOYINQIDEYANGPINBIANXINGKONGZHIZAIYIDINGDEFANWEINEI。SHANGSHU 5 ZHONGGANZAOFANGFAGEYOUYOUQUEDIAN,QIEGEYOUSHIYONGDESHENGWUYANGPINZHONGLEI。WOMENZAIXUANZESHENGWUYANGPINGANZAODEFANGFASHI,JINLIANGXUANZENENGBAOCHIYANGPINBIAOMIANZHENSHIJIEGOUDEFANGFA,ZAIBAOZHENGSAOMIAODIANJINGZHUANGTAIXIA,JINLIANGHAIYUANYANGPINBIAOMIANXIJIE。

 

DUIYUSHENGWUYANGPIN,FEINADIANJINGDIZHENKONGMOSHIKEYIZHIJIEGUANCHAYIXIESHENGWUYANGPIN,RUXINXIANZHIWUYEPIAN,KUNCHONGDENG。GUANCHAYEZIQIKONG,GAOLIANGDUDE CeB6 DENGSIKEYIZAIDIPUGUANGSHIJIANXIAKUAISUPAIZHAO,HUODEGAOZHILIANGTUXIANG,BIMIANLEZHANGSHIJIANPUGUANGDAOZHIQIKONGGUANBIYIJIZHANGSHIJIANCHOUZHENKONGDAOZHIYEPIANSHISHUISHOUSUO。

 

   

滚球体育FEINADIANJINGDIZHENKONGXIAGUANCHAYEZIQIKONG

 

CIWAI,FEINADIANJINGDEZHUANYONGKONGWENYANGPINBEIKEYIKONGZHIYANGPINWENDU,JINERGAIBIANYANGPINZHOUWEIDESHIDU,DAXIANDUDIJIANGDIDIANZISHUHEZHENKONGDUDUIYANGPINDESUNHUAI。SHIYONGYUYIXIEHANSHUIJIAOGAODESHUININGJIAO,DANBAI,ZAOLEIDENGYANGPIN,ZAILENGDONGZHUANGTAIXIA,BUTUOSHUI,BEISANSHEZHIJIEGUANCHA,KEYIBAOCHIYANGPINDEYUANSHIXINGMAOJIEGOU。

 

    

LENGDONGDESHUININGJIAO,GUANCHADAOQIDAFANWEINEI,DUANCHENGYOUXU,ZHANGCHENGWUXU

滚球体育CHUANZHEN:86-021-28986356

邮箱:info@phenom-china.com

滚球体育DIZHI:SHANGHAISHIPUDONGXINQUZHANGJIANGGAOKEJIYUANQUKEYUANLU88HAODEGUOZHONGXINEZUO502

版权所有 © 2018 复纳科学仪器(上海)有限公司   备案号:    技术支持:化工仪器网  GoogleSitemap

联系人
在线客服
扫码关注我们
用心服务 成就你我
访问手机站